14.7 Folate:B12

14.7.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 231.605099 1 <0.0001 ***
folate_B12 4.066344 1 0.04 *
Study 1.512595 1 0.2 ns
GA_samp 175.975135 1 <0.0001 ***
folate_B12:Study 4.357133 1 0.04 *
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 43.8435148 1 <0.0001 ***
folate_B12 0.1148209 1 0.7 ns
Study 12.6704561 1 0.0004 ***
GA_samp 2499.8832090 1 <0.0001 ***
folate_B12:Study 0.1766640 1 0.7 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 411.4212786 1 <0.0001 ***
folate_B12 5.6849187 1 0.02 *
Study 0.2822938 1 0.6 ns
GA_samp 1075.9312193 1 <0.0001 ***
folate_B12:Study 0.3248007 1 0.6 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 folate_B12 1 Inf 0.311 0.311 0.6 ns
3 Study 1 Inf 4.120 4.120 0.04 *
4 GA_samp 1 Inf 1.038 1.038 0.3 ns
2 folate_B12:Study 1 Inf 1.565 1.565 0.2 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 folate_B12 1 Inf 0.405 0.405 0.5 ns
3 Study 1 Inf 12.419 12.419 0.0004 ***
4 GA_samp 1 Inf 0.532 0.532 0.5 ns
2 folate_B12:Study 1 Inf 1.602 1.602 0.2 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 folate_B12 1 Inf 0.680 0.680 0.4 ns
3 Study 1 Inf 85.551 85.551 <0.0001 ***
4 GA_samp 1 Inf 0.586 0.586 0.4 ns
2 folate_B12:Study 1 Inf 0.108 0.108 0.7 ns

14.7.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 15.83 (11.09, 22.58) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.04
StudySTOP 0.93 (0.82, 1.05) 0.22
GA_samp 1.15 (1.13, 1.18) <0.0001
folate_B12:StudySTOP 1.00 (1.00, 1.00) 0.04
hPL (μg/ml)
Var Effect P
(Intercept) 1.87 (1.56, 2.25) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.35
StudySTOP 1.12 (1.06, 1.18) 0.0001
GA_samp 1.34 (1.32, 1.36) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.08 (0.06, 0.10) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.004
StudySTOP 0.99 (0.92, 1.07) 0.78
GA_samp 1.30 (1.28, 1.32) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 12.40 (7.86, 19.57) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.50
StudySTOP 0.79 (0.69, 0.92) 0.002
GA_samp 1.02 (0.99, 1.05) 0.30
HOMA-IR
Var Effect P
(Intercept) 3.17 (1.96, 5.13) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.46
StudySTOP 0.68 (0.59, 0.80) <0.0001
GA_samp 1.01 (0.98, 1.04) 0.45
Glucose (mmol/L)
Var Effect P
(Intercept) 5.69 (5.21, 6.22) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.37
StudySTOP 0.86 (0.84, 0.88) <0.0001
GA_samp 1.00 (0.99, 1.00) 0.45

14.7.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Folate:B12 ratio and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 97.53239 7.251682 83.62721 84.12625 113.0749
STOP 96.68844 6.873838 70.00547 83.90659 111.4174
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 88.43035 2.320844 144.0892 83.96001 93.13872
STOP 98.82664 2.377019 101.8720 94.22247 103.65580
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.332928 0.0639365 389.4782 2.210551 2.462080
STOP 2.308206 0.0528096 219.2253 2.206439 2.414668
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 14.96949 0.7247602 Inf 13.61430 16.45958
STOP 11.89513 0.4308389 Inf 11.07997 12.77025
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.637941 0.1871675 Inf 3.288988 4.023916
STOP 2.490395 0.0951873 Inf 2.310648 2.684124
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.496623 0.0508714 Inf 5.397816 5.597239
STOP 4.729527 0.0347387 Inf 4.661929 4.798107

14.7.4 Comparisons

Effect for every 100 unit increase in Folate:B12 ratio.
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) folate_B12245.96 / folate_B12145.96 SCOPE 0.98 (0.95, 1.00) 0.04
PRL (ng/ml) folate_B12245.96 / folate_B12145.96 STOP 1.01 (0.99, 1.03) 0.4
hPL (μg/ml) folate_B12245.96 / folate_B12145.96 SCOPE 1.00 (0.99, 1.00) 0.4
hPL (μg/ml) folate_B12245.96 / folate_B12145.96 STOP 1.00 (0.99, 1.00) 0.4
GH2 (ng/ml) folate_B12245.96 / folate_B12145.96 SCOPE 0.98 (0.97, 0.99) 0.004
GH2 (ng/ml) folate_B12245.96 / folate_B12145.96 STOP 0.98 (0.97, 0.99) 0.004
Insulin (uU/ml) folate_B12245.96 / folate_B12145.96 SCOPE 0.99 (0.97, 1.01) 0.5
Insulin (uU/ml) folate_B12245.96 / folate_B12145.96 STOP 0.99 (0.97, 1.01) 0.5
HOMA-IR folate_B12245.96 / folate_B12145.96 SCOPE 0.99 (0.97, 1.01) 0.5
HOMA-IR folate_B12245.96 / folate_B12145.96 STOP 0.99 (0.97, 1.01) 0.5
Glucose (mmol/L) folate_B12245.96 / folate_B12145.96 SCOPE 1.00 (0.99, 1.00) 0.4
Glucose (mmol/L) folate_B12245.96 / folate_B12145.96 STOP 1.00 (0.99, 1.00) 0.4

14.7.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.7.6 Plots